Autoradiographs of Pollen Tube Nuclei with Calcium-45

Calcium accumulations within the growing tips of pollen tubes

Pollen of L. longiflorum was grown in 45Ca-labeled medium and washed with nonradioactive medium. Whole, labeled pollen was then frozen and autoradiographed at -78 degrees C. The autoradiographs show striking accumulations of 45Ca in the growing tips of the pollen tubes. This result is obtained when the pollen is labeled for times as short as 1 min, or as long as 5 h. In most cases, the tip conc...

Fast pollen tube growth in Conospermum species.

BACKGROUND AND AIMS An unusual form of pollen tube growth was observed for several Conospermum species (family Proteaceae). The rate of pollen tube growth, the number of tubes to emerge and the ultrastructure of these tubes are given here. METHODS Pollen was germinated in vitro in different sucrose concentrations and in the presence of calcium channel blockers, and tube emergence and growth w...

Title: A Calcium-Dependent Protein Kinase Interacts with and Activates A Calcium Channel to Regulate Pollen Tube Growth Running Title: CPK32-CNGC18 Interaction and Pollen Tube Growth

Overexpression of PwTUA1, a pollen-specific tubulin gene, increases pollen tube elongation by altering the distribution of α-tubulin and promoting vesicle transport

Tubulin genes are intimately associated with cell division and cell elongation, which are central to plant secondary cell wall development. However, their roles in pollen tube polar growth remain elusive. Here, a TUA1 gene from Picea wilsonii, which is specifically expressed in pollen, was isolated. Semi-quantitative RT-PCR analysis showed that the amount of PwTUA1 transcript varied at each sta...

Self-incompatibility in the Iranian Almond Cultivar ‘Mamaei’ Using Pollen Tube Growth, Fruit Set and PCR Technique

Self-incompatibility has been studied by using controlled pollination, pollen tube growth and PCR methods in the Iranian almond ‘Mamaei.’. Pollen tube growth and fruit set following self and cross-pollination treatments were evaluated. The percentage of initial and final fruit set was determined for each treatment at 30 and 60 days after controlled pollination. Pollen germination and pollen ...